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@ ewok
2025-01-09 01:31:41
**Sanger sequencing traces.** I extracted DNA directly from my cells and determined their sequence, which is visualized by the bottom trace. In a second sample, I mutated my DNA enzymatically and used to same process to determine the sequence, shown in the top trace. Each peak and color represents a unique DNA base (A, T, C or G) in part of a gene with 13 bases shown here in total. This biogenerative process resulted in the center base changing from T to C, resulting in a slightly different, mixed peak by comparison. To further separate this work from being purely scientific, I did not record specific details about the experimental process.
<img src="https://i.nostr.build/oSgJNXy2dAmZ520S.jpg">